PreLab-- TLC and Column Chromatography

Prelab

Lab #5: TLC and Column Chromatography

Chemicals	Hazards	MW	Grams	Density	Moles
Hexane	Don’t ingestion. Irritates skin and eye contact.	86.17536 g/mol	9.0 ml	655 kg/m³	0.07 mol
Acetone	Irritate eye and skin	58.08 g/mol	2.0 ml	791 kg/m³	0.03 mol
Alumina	Don’t ingestion. Irritates skin and eye contact.	101.96 g/mol	1.25 g	3.95 g/cm³	0.013 mol
Fluorene	Irritate eye and skin	166.22 g/mol	0.3 ml	1.2 g/cm³	0.002 mol
Fluorenone	Irritate eye and skin	180.192 g/mol	0.3 ml	1.13 g/cm³	0.0017 mol

Procedure

1. Take four test tube and two Pasteur pipets
2. Place 9 ml hexane, 2 ml acetone and 2 ml 70% hexane-30% acetone in 3 Erlenmeyer flasks.
3. Place 0.3 ml of step 2 solution.
4. Prepare 4 micropipets.
5. Prepare a chromatography column with alumina.
6. Place cotton in the Pasteur pipet.
7. Add 1.25 g of alumina to the pipet
8. Place test tube 1 under the column.
9. Add 3 ml of hexane to the column
10. Add the solution of Fluorene and Fluorenone.
11. Collect the eluent in test tube two.
12. Add 1ml of hexane drain until the surface reached THE ALUMINA.
13. Add 5 ml of hexane.
14. Use acetone to clean solid Fluorene off the pipet.
15. Place test tube 3 under the column before the yellow band reaches the bottom of the column.
16. Test the purities of the fluorenone and fluorene.
17. Spot the solution several times.
18. Spot the two reference solution containing fluorene and fluorenone.
19. Evaporate the solvent from test tubes 2 and 3 in a warm bath.
20. Determine the melting point.