TLC and Column Chromatography- Post Lab 5



Observations:
1.      Fluorene traveled further on the TLC plate then the fluorenone.
2.      The samples on the TLC plate were not visible with eyes.
3.      After 70% hexane – 30% acetone was added, the yellow band went down faster.
Data:
Test tube 3 had the fluorenone, test tube 2 had fluorene and the dot label “M” is a mixture of fluorenone and fluorene. Fluorene travel up more on the TLC plate than fluorenone.

Post-lab Question: 
Why does fluorenone travel more slowly than fluorene? Why does acetone speed up the rate at which fluorenone travels?
Fluorenone has a carbonyl group which means that it is more polar than fluorene. Since fluorenone is more polar it travel more slowly than fluorene. Acetone will speed up the rate at which both fluorene andfluorenone travels up the TLC plate. It will not change the position.

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